Enzyme Technology and Industrial Applications

BT 9252     ENZYME TECHNOLOGY AND INDUSTRIAL APPLIC ATIONS  

UNIT I   KINETICS AND MECHANISM OF ENZYME ACTION             8
Classification  of  enzymes;  quantification  of  enzyme  activity  and  specific  activity.
Estimation of  Michaelis Menten parameters,  Effect of pH  and  temperature  on enzyme
activity, kinetics of inhibition. Modeling of rate equations for single and multiple substrate
reactions.
UNIT II   IMMOBILISED ENZYME REACTIONS                   9
Techniques of enzyme immobilisation-matrix entrapment, ionic and cross linking, column
packing; Analysis of mass transfer effects of kinetics of immobilised enzyme reactions;
Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized enzyme reactions;
calculation  of  Effectiveness  Factors  of  immobilized  enzyme  systems;  Bioconversion
studies with immobilized enzyme packed -bed reactors.
UNIT III  MASS TRANSFER EFFECTS IN IMMOBILISED   ENZYME SYSTEMS
5
Analysis of film and Pore diffusion Effects on kinetics of immobilised enzyme reactions;
Formulation of dimensionless groups and calculation of Effectiveness Factors
UNIT IV  APPLICATION OF ENZYMES                 12
Extraction  of  commercially  important  enzymes  from  natural  sources;  Commercial
applications  of  enzymes  in  food,  pharmaceutical  and  other  industries;  enzymes  for
diagnostic applications.  Industrial  production of enzymes.  Use  of enzymes in analysis-
types  of  sensing-gadgetry  and  methods.    Case  studies  on  application    -  chiral
conversion, esterification etc.,
UNIT V  ENZYME BIOSENSORS                   11
Applications of enzymes in analysis; Design of enzyme electrodes and case studies on
their application as biosensors in industry, healthcare and environment.
TOTAL:  45 PERIODS
REFERENCES:
1.Blanch, H.W.,  Clark, D.S.  Biochemical Engineering, Marcel Dekker, 1997
2.Lee, James M. Biochemical Engineering, PHI, USA.
3.Bailey  J.E. & Ollis, D.F. Biochemical Engineering Fundamentals,McGrawHill, 1986
4. Wiseman, Alan. Hand book of  Enzyme Biotechnology, Ellis  Harwood 1995.


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